Reference · GR-C-TBD 045
– AMOXICILLIN
- Category
- Consumables
- Price
- F CFA 12,000
Certifications
IVD (For In Vitro Diagnostic Use)GMP (Good Manufacturing Practices Certified)
Disc diffusion test is a qualitative test method. This method is based on the principle that antibiotic-impregnated disk,
placed on agar previously inoculated with the test bacterium, picks up moisture and the antibiotic diffuse radially outward
through the agar medium producing an antibiotic concentration gradient. The concentration of the antibiotic at the edge
of the disk is high and gradually diminishes as the distance from the disk increases to a point where it is no longer
inhibitory for the organism, which then grows freely. A clear zone or ring is formed around an antibiotic disk after
incubation if the agent inhibits bacterial growth. The disk diffusion method is performed using Mueller-Hinton Agar
(MHA), which is the best medium for routine susceptibility tests because it has good reproducibility, low in sulfonamide,
trimethoprim, and tetracycline inhibitors, and gives satisfactory growth of most bacterial pathogens
Technical specifications
| The agar depth should be 4.0 ± 0.5 mm. | |
|---|---|
| The inoculum suspension should optimally be used within 15 and always within 60 min of preparation to ensure | |
| the correct number of viable cells. | |
| During inoculation, prohibit the use of flooding the plates. | |
| Antibiotic disks should be applied within 15 min of inoculation and start incubation within another 15 minutes. | |
| Do not to exceed the incubation period of 16–20 h, because prolonged incubation often results in indistinct zone | |
| edges or colonies within the inhibition zones, which might produce false results. | |
| A maximum of six disks can be accommodated on a 90-mm circular plate and 12 on a 150-mm circular plate. |